Immunolocalization of Atrial Natriuretic Peptide and C-Type Natriuretic Peptide In Healthy and Pre-Eclamptic Human Placenta
Öz
Pregnancy triggers
extensive alterations in the maternal vasculature to deliver a constant supply
of blood to the growing fetus. Natriuretic peptides are hormones secreted by many
organs. Preeclampsia is associated with increased maternal plasma natriuretic
peptide levels, which are used as novel biochemical markers to predict the
preeclampsia. Here, we aimed to determine the immunohistochemical distribution
of atrial natriuretic peptide (ANP) and C-type natriuretic peptide (CNP) in
normal and pre-eclamptic human placentas. Tissue samples from maternal and
fetal parts of pre-eclamptic and healthy human placentas were subjected to
routine tissue processing. Avidin-biotin-peroxidase complex (ABC) immunohistochemistry
was applied to formalin-fixed, paraffin-embedded placenta sections collected
onto poly-l-lysine slides to demonstrate ANP and CNP expression.
Immunohistochemistry revealed immunoreactivity for ANP and CNP in fetal and maternal
tissues. On the fetal side of the preeclampsia group, strong ANP and CNP
immunoreactivity were detected in the amniotic epithelium, fetal vessels,
villous syncytiotrophoblast plate, villous vascular endothelial cells and
smooth muscle cells than the healthy group. On the maternal side of the
pre-eclampsia group, decidual cells exhibited lower ANP and CNP
immunoreactivity than healthy group. These data indicate that increased ANP and
CNP expression relating to preeclampsia might serve as a compensatory function
to properly maintain uteroplacental perfusion and fetal development during
pregnancy.
Anahtar Kelimeler
Preeclampsia Atrial Natriuretic Peptide C-Type Natriuretic Peptide Placenta Pregnancy
Kaynakça
- Boeldt DS, Bird IM (2017). Vascular adaptation in pregnancy and endothelial dysfunction in preeclampsia. J Endocrinol, 232(1), 27-44.
- Brown MA (1995). The physiology of pre-eclampsia. Clin Exp Pharmacol Physiol, 22(11), 781-791.
- Cameron VA, Aitken GD, Ellmers LJ, Kennedy MA, Espiner EA (1996). The sites of gene expression of atrial, brain, and C-type natriuretic peptides in mouse fetal development: temporal changes in embryos and placenta. Endocrinology, 137(3), 817-824.
- Cartwrighta JE, Kenny LC, Dash PR, Crocker IP, Aplin JD, Baker PN, Whitley GS (2002). Trophoblast invasion of spiral arteries: a novel in vitro model. Placenta, 23(2), 232-235.
- Casco VH, Veinot JP, De Bold MLK, Masters RG, Stevenson MM, de Bold AJ (2002). Natriuretic peptide system gene expression in human coronary arteries. J Histochem Cytochem, 50(6), 799-809.
- Ghuman N, Rheiner J, Tendler BE, White WB (2009). Hypertension in the postpartum woman: clinical update for the hypertension specialist. J Clin Hypertens, 11(12), 726-733.
- Giannubilo SR, Pasculli A, Tidu E, Biagini A, Boscarato V, Ciavattini A (2017). Relationship between maternal hemodynamics and plasma natriuretic peptide concentrations during pregnancy complicated by preeclampsia and fetal growth restriction. J Perinatol, 37(5), 484-487.
- Graham CH, Watson JD, Blumenfeld AJ, Pang SC (1996). Expression of atrial natriuretic peptide by third-trimester placental cytotrophoblasts in women. Biol Reprod, 54(4), 834-840.
- Greer IA, Haddad NG, Dawes J (1989). Neutrophil activation in pregnancy induced hypertension. Br J Obstet Gynaecol, 96(8), 978-982.
- Gude NM, Stebbing PN, Wang L, Xue J, Brennecke SP, Lim AT (2000). Relative abundance of placental pro-atrial natriuretic factor mRNA in normal pregnancy and pre-eclampsia. Gynecol Obstet Invest, 49(2), 114-118.
- Harris LK (2011). IFPA gabor than award lecture: transformation of the spiral arteries in human pregnancy: key events in the remodelling timeline. Placenta, 32(Suppl 2), 154-158.
- Huang W, Lee D, Yang Z, Casley D, Throsby M, Copolov DL, Johnston C, Lim AT (1992). Evidence for atrial natriuretic peptide-(5-28) production by rat placental cytotrophoblasts. Endocrinology, 131(2), 919-924.
- Inglis GC, Kingdom JC, Nelson DM, Lindop GB, Whittle MJ, Connell JM (1993). Atrial natriuretic hormone: a paracrine or endocrine role within the human placenta. IJ Clin Endocrinol Metab, 76(4), 1014-1018.
- Ishizaka Y, Kangawa K, Minamino N, Ishii K, Takano S, Eto T, Matsuo H (1992). Isolation and identification of C-type natriuretic peptide in human monocytic cell line, THP-1. Biochem Biophys Res Commun, 189(2), 697-704.
- Itoh H, Sagawa N, Hasegawa M, Nanno H, Kobayashi F, Ihara Y, Mori T, Komatsu Y, Suga S, Yoshimasa T (1994). Expression of biologically active receptors for natriuretic peptides in the human uterus during pregnancy. Biochem Biophys Res Commun, 203(1), 602-607.
- Ivanova MD, Gregoraszczuk EL, Augustowska K, Kolodziejczyk J, Mollova MV, Kehayov IR (2003). Localization of atrial natriuretic peptide in pig granulosa cells isolated from ovarian follicles of various size. Reprod Biol, 3(2), 173-181.
- Kelsall CJ, Chester AH, Sarathchandra P, Singer DR (2006). Expression and localization of C-type natriuretic peptide in human vascular smooth muscle cells. Vascul Pharmacol, 45(6), 368-373.
- Keman K, Prasetyorini N, Langgar MJ (2009). Perbandingan eksprei P53, Bcl-2, dan indeks apoptosis trofoblas pada preeklampsia/eklampsia dan kehamilan normal. INAJOG, 33(3), 151-159.Khan KS, Wojdyla D, Say L, Gulmezoglu AM, Van Look PF (2006). WHO analysis of causes of maternal death: a systematic review. Lancet, 367(9516), 1066-1074.
- Lim AT, Gude NM (1995). Atrial natriuretic factor production by the human placenta. J Clin Endocrinol Metab, 80(10), 3091-3093.
- Lumme R, Laatikainen T, Vuolteenaho O (1992). Plasma endothelin, atrial natriuretic peptide (ANP) and uterine and umbilical artery flow velocity waveforms in hypertensive pregnancies. Br J Obstet Gynaecol, 99(9), 761-764.
- Madhavan S, Prickett TCR, Espiner EA, Barrell, GK (2017). Nutrient restriction in early ovine pregnancy stimulates C-type natriuretic peptide production. Reprod Fertil Dev, 29(3), 575-584.
- McNeill BA, Barrell GK, Ridgway MJ, Wellby MP, Prickett TC, Espiner EA (2012). Caloric restriction, but not caloric loading, affects circulating fetal and maternal C-type natriuretic peptide concentrations in late ovine gestation. Reprod Fertil Dev, 24(8), 1063-1070.
- McNeill BA, Barrell GK, Wellby M, Prickett TC, Yandle TG, Espiner EA (2009). C-type natriuretic peptide forms in pregnancy: maternal plasma profiles during ovine gestation correlate with placental and fetal maturation. Endocrinology, 50(10), 4777-4783.
- Minegishi T, Nakamura M, Abe K, Tano M, Andoh A, Yoshida M, Takagi T, Nishikimi T, Kojima M, Kangawa K (1999). Adrenomedullin and atrial natriuretic peptide concentrations in normal pregnancy and pre-eclampsia. Mol Hum Reprod, 5(8), 767-770.
- Naruko T, Ueda M, van der Wal AC, van der Loos CM, Itoh H, Nakao K, Becker AE (1996). C-type natriuretic peptide in human coronary atherosclerotic lesions. Circulation, 94(12), 3103-3108.
- Rampersad R, Cervar-Zivkovic M, Nelson DM (2011). Development and anatomy of the human placenta. In: The Placenta: From Development to Disease, Kay H, Nelson DM, Wang Y (Ed), 19-26, Wiley-Blackwell, United Kingdom.
- Redman CW, Sargent IL (2005). Latest advances in understanding pre-eclampsia. Science, 308(5728), 1592-1594.
- Reid RA, Prickett TC, Pullar BE, Darlow BA, Gullam JE, Espiner EA (2014). C-type natriuretic peptide in complicated pregnancy: increased secretion precedes adverse events. J Clin Endocrinol Metab, 99(4), 1470-1478.
- Sagnella GA, Markandu ND, Shore AC, MacGregor GA (1986). Raised circulating levels of atrial natriuretic peptides in essential hypertension. Lancet, 1(8474), 179-181.
- Scott JN (1993). Atrial natriuretic peptide binding in rat placenta, yolk sac, decidua, and maternal placental vessels. Cell Tissue Res, 272(2), 391-394.
- Sellitti DF, Koles N, Mendonca MC (2011). Regulation of C-type natriuretic peptide expression. Peptides, 32(9), 1964-1971.
- Stepan H, Faber R, Stegemann S, Schultheiss HP, Walther T (2002). Expression of C-type natriuretic peptide in human placenta and myometrium in normal pregnancies and pregnancies complicated by intrauterine growth retardation. Preliminary results. Fetal Diagn Ther, 17(1), 37-41.
- Suga S, Itoh H, Komatsu Y, Ogawa Y, Hama N, Yoshimasa T, Nakao K (1993). Cytokine-induced C-type natriuretic peptide (CNP) secretion from vascular endothelial cells - evidence for CNP as a novel autocrine/paracrine regulator from endothelial cells. Endocrinology, 133(6), 3038-3041.
- Thomsen JK, Storm TL, Thamsborg G, Nully M, Bodker B, Skouby S (1987). Atrial natriuretic peptide concentrations in pre-eclampsia. Br Med J, 294(6586), 1508-1510.
- Wang W, Dong N, Lou J, Srinivasan DK, Cheng W, Huang X, Liu M, Fang C, Peng J, Chen S, Wu S, Liu Z, Dong L, Zhou Y, Wu Q (2012). Role of corin in trophoblast invasion and uterine spiral artery remodelling in pregnancy. Nature, 484(7393), 246-250.
- Zhou Y, Wu Q (2013). Role of corin and atrial natriuretic peptide in preeclampsia. Placenta, 34(2), 89-94.
Sağlıklı ve Pre-Eklampsili İnsan Plasentasında Atrial Natriüretik Peptid ve C-Tip Natriüretik Peptidin İmmunolokalizasyonu
Öz
Gebelik, büyümekte olan
fötüsün sürekli kan ihtiyacının karşılanması için maternal damar sisteminde
önemli değişimlerin meydana gelmesini tetiklemektedir. Natriüretik peptidler, birçok
organ tarafından salgılanan hormonlardır. Pre-eklampsi, maternal plazma
natriüretik peptid seviyelerindeki artış ile ilişkilidir ve plazma natriüretik
peptid düzeyleri, pre-eklampsinin tahmin edilmesinde yeni biyokimyasal belirteçler
olarak kullanılmaktadır. Burada sağlıklı ve pre-eklampsili insan plasentalarında
atrial natriüretik peptid (ANP) ve C-tip natriüretik peptidin (CNP) immunohistakimyasal
dağılımını belirlemeyi amaçladık. Pre-eklampsili ve sağlıklı insan plasentalarının
maternal ve fötal kısımlarından alınan doku örnekleri rutin doku işleme sürecinden
geçirilmiştir. ANP ve CNP ekspresyonunu göstermek için poli-lizinli lamlar
üzerine alınan kesitlere avidin-biyotin-peroksidaz kompleks yöntemi uygulanmıştır.
İmmunohistokimya, fetal ve maternal dokulardaki ANP ve CNP immunoreaktivitesini
ortaya çıkarmıştır. Pre-eklampsili grubun fötal tarafındaki amniyon epitelinde,
fötal kan damarlarında, villöz sinsitiyotrofoblast hücrelerinde, villöz damar
endotel hücrelerinde ve düz kas hücrelerinde sağlıklı gruba göre güçlü ANP ve
CNP immunoreaktivitesi saptanmıştır. Maternal tarafındaki desidual hücrelerde
ise sağlıklı grubuna nazaran daha zayıf ANP ve CNP immunoreaktivitesi gözlendi.
Bu sonuçlar, pre-eklampsili ile ilgili olarak artan ANP ve CNP ekspresyonunun
gebelik sürecinde utero-plasental perfüzyon ve fötal gelişimin uygun bir
şekilde devam etmesi için telafi edici bir fonksiyon görebileceğini akla getirmektedir.
Anahtar Kelimeler
Pre-eklampsi Atrial Natriüretik Peptid C-Tip Natriüretik Peptid Plasenta Gebelik
Kaynakça
- Boeldt DS, Bird IM (2017). Vascular adaptation in pregnancy and endothelial dysfunction in preeclampsia. J Endocrinol, 232(1), 27-44.
- Brown MA (1995). The physiology of pre-eclampsia. Clin Exp Pharmacol Physiol, 22(11), 781-791.
- Cameron VA, Aitken GD, Ellmers LJ, Kennedy MA, Espiner EA (1996). The sites of gene expression of atrial, brain, and C-type natriuretic peptides in mouse fetal development: temporal changes in embryos and placenta. Endocrinology, 137(3), 817-824.
- Cartwrighta JE, Kenny LC, Dash PR, Crocker IP, Aplin JD, Baker PN, Whitley GS (2002). Trophoblast invasion of spiral arteries: a novel in vitro model. Placenta, 23(2), 232-235.
- Casco VH, Veinot JP, De Bold MLK, Masters RG, Stevenson MM, de Bold AJ (2002). Natriuretic peptide system gene expression in human coronary arteries. J Histochem Cytochem, 50(6), 799-809.
- Ghuman N, Rheiner J, Tendler BE, White WB (2009). Hypertension in the postpartum woman: clinical update for the hypertension specialist. J Clin Hypertens, 11(12), 726-733.
- Giannubilo SR, Pasculli A, Tidu E, Biagini A, Boscarato V, Ciavattini A (2017). Relationship between maternal hemodynamics and plasma natriuretic peptide concentrations during pregnancy complicated by preeclampsia and fetal growth restriction. J Perinatol, 37(5), 484-487.
- Graham CH, Watson JD, Blumenfeld AJ, Pang SC (1996). Expression of atrial natriuretic peptide by third-trimester placental cytotrophoblasts in women. Biol Reprod, 54(4), 834-840.
- Greer IA, Haddad NG, Dawes J (1989). Neutrophil activation in pregnancy induced hypertension. Br J Obstet Gynaecol, 96(8), 978-982.
- Gude NM, Stebbing PN, Wang L, Xue J, Brennecke SP, Lim AT (2000). Relative abundance of placental pro-atrial natriuretic factor mRNA in normal pregnancy and pre-eclampsia. Gynecol Obstet Invest, 49(2), 114-118.
- Harris LK (2011). IFPA gabor than award lecture: transformation of the spiral arteries in human pregnancy: key events in the remodelling timeline. Placenta, 32(Suppl 2), 154-158.
- Huang W, Lee D, Yang Z, Casley D, Throsby M, Copolov DL, Johnston C, Lim AT (1992). Evidence for atrial natriuretic peptide-(5-28) production by rat placental cytotrophoblasts. Endocrinology, 131(2), 919-924.
- Inglis GC, Kingdom JC, Nelson DM, Lindop GB, Whittle MJ, Connell JM (1993). Atrial natriuretic hormone: a paracrine or endocrine role within the human placenta. IJ Clin Endocrinol Metab, 76(4), 1014-1018.
- Ishizaka Y, Kangawa K, Minamino N, Ishii K, Takano S, Eto T, Matsuo H (1992). Isolation and identification of C-type natriuretic peptide in human monocytic cell line, THP-1. Biochem Biophys Res Commun, 189(2), 697-704.
- Itoh H, Sagawa N, Hasegawa M, Nanno H, Kobayashi F, Ihara Y, Mori T, Komatsu Y, Suga S, Yoshimasa T (1994). Expression of biologically active receptors for natriuretic peptides in the human uterus during pregnancy. Biochem Biophys Res Commun, 203(1), 602-607.
- Ivanova MD, Gregoraszczuk EL, Augustowska K, Kolodziejczyk J, Mollova MV, Kehayov IR (2003). Localization of atrial natriuretic peptide in pig granulosa cells isolated from ovarian follicles of various size. Reprod Biol, 3(2), 173-181.
- Kelsall CJ, Chester AH, Sarathchandra P, Singer DR (2006). Expression and localization of C-type natriuretic peptide in human vascular smooth muscle cells. Vascul Pharmacol, 45(6), 368-373.
- Keman K, Prasetyorini N, Langgar MJ (2009). Perbandingan eksprei P53, Bcl-2, dan indeks apoptosis trofoblas pada preeklampsia/eklampsia dan kehamilan normal. INAJOG, 33(3), 151-159.Khan KS, Wojdyla D, Say L, Gulmezoglu AM, Van Look PF (2006). WHO analysis of causes of maternal death: a systematic review. Lancet, 367(9516), 1066-1074.
- Lim AT, Gude NM (1995). Atrial natriuretic factor production by the human placenta. J Clin Endocrinol Metab, 80(10), 3091-3093.
- Lumme R, Laatikainen T, Vuolteenaho O (1992). Plasma endothelin, atrial natriuretic peptide (ANP) and uterine and umbilical artery flow velocity waveforms in hypertensive pregnancies. Br J Obstet Gynaecol, 99(9), 761-764.
- Madhavan S, Prickett TCR, Espiner EA, Barrell, GK (2017). Nutrient restriction in early ovine pregnancy stimulates C-type natriuretic peptide production. Reprod Fertil Dev, 29(3), 575-584.
- McNeill BA, Barrell GK, Ridgway MJ, Wellby MP, Prickett TC, Espiner EA (2012). Caloric restriction, but not caloric loading, affects circulating fetal and maternal C-type natriuretic peptide concentrations in late ovine gestation. Reprod Fertil Dev, 24(8), 1063-1070.
- McNeill BA, Barrell GK, Wellby M, Prickett TC, Yandle TG, Espiner EA (2009). C-type natriuretic peptide forms in pregnancy: maternal plasma profiles during ovine gestation correlate with placental and fetal maturation. Endocrinology, 50(10), 4777-4783.
- Minegishi T, Nakamura M, Abe K, Tano M, Andoh A, Yoshida M, Takagi T, Nishikimi T, Kojima M, Kangawa K (1999). Adrenomedullin and atrial natriuretic peptide concentrations in normal pregnancy and pre-eclampsia. Mol Hum Reprod, 5(8), 767-770.
- Naruko T, Ueda M, van der Wal AC, van der Loos CM, Itoh H, Nakao K, Becker AE (1996). C-type natriuretic peptide in human coronary atherosclerotic lesions. Circulation, 94(12), 3103-3108.
- Rampersad R, Cervar-Zivkovic M, Nelson DM (2011). Development and anatomy of the human placenta. In: The Placenta: From Development to Disease, Kay H, Nelson DM, Wang Y (Ed), 19-26, Wiley-Blackwell, United Kingdom.
- Redman CW, Sargent IL (2005). Latest advances in understanding pre-eclampsia. Science, 308(5728), 1592-1594.
- Reid RA, Prickett TC, Pullar BE, Darlow BA, Gullam JE, Espiner EA (2014). C-type natriuretic peptide in complicated pregnancy: increased secretion precedes adverse events. J Clin Endocrinol Metab, 99(4), 1470-1478.
- Sagnella GA, Markandu ND, Shore AC, MacGregor GA (1986). Raised circulating levels of atrial natriuretic peptides in essential hypertension. Lancet, 1(8474), 179-181.
- Scott JN (1993). Atrial natriuretic peptide binding in rat placenta, yolk sac, decidua, and maternal placental vessels. Cell Tissue Res, 272(2), 391-394.
- Sellitti DF, Koles N, Mendonca MC (2011). Regulation of C-type natriuretic peptide expression. Peptides, 32(9), 1964-1971.
- Stepan H, Faber R, Stegemann S, Schultheiss HP, Walther T (2002). Expression of C-type natriuretic peptide in human placenta and myometrium in normal pregnancies and pregnancies complicated by intrauterine growth retardation. Preliminary results. Fetal Diagn Ther, 17(1), 37-41.
- Suga S, Itoh H, Komatsu Y, Ogawa Y, Hama N, Yoshimasa T, Nakao K (1993). Cytokine-induced C-type natriuretic peptide (CNP) secretion from vascular endothelial cells - evidence for CNP as a novel autocrine/paracrine regulator from endothelial cells. Endocrinology, 133(6), 3038-3041.
- Thomsen JK, Storm TL, Thamsborg G, Nully M, Bodker B, Skouby S (1987). Atrial natriuretic peptide concentrations in pre-eclampsia. Br Med J, 294(6586), 1508-1510.
- Wang W, Dong N, Lou J, Srinivasan DK, Cheng W, Huang X, Liu M, Fang C, Peng J, Chen S, Wu S, Liu Z, Dong L, Zhou Y, Wu Q (2012). Role of corin in trophoblast invasion and uterine spiral artery remodelling in pregnancy. Nature, 484(7393), 246-250.
- Zhou Y, Wu Q (2013). Role of corin and atrial natriuretic peptide in preeclampsia. Placenta, 34(2), 89-94.
Ayrıntılar
| Birincil Dil | İngilizce |
|---|---|
| Konular | Veteriner Cerrahi |
| Bölüm | Makaleler |
| Yazarlar | |
| Yayımlanma Tarihi | 25 Mart 2019 |
| Gönderilme Tarihi | 25 Temmuz 2018 |
| Kabul Tarihi | 22 Kasım 2018 |
| Yayımlandığı Sayı | Yıl 2019 Cilt: 30 Sayı: 1 |
Kaynak Göster
Kabul edilen makaleler Creative Commons Atıf-Ticari Olmayan Lisansla Paylaş 4.0 uluslararası lisansı ile lisanslanmıştır.